Instructions for collecting material

Collecting semen for clinical and microbiological examination.

There is no special room in the E.Gulbis Laboratory for collecting semen.

The semen can be collected:

a) At home.
b) In the Embrions Ltd., in Riga Tālivalža street 2a, arranging an appointment beforehand by phone 67543350 (additional fee 5 EUR).

  1. Before collecting semen for clinical testing the patient has to refrain from sex life for 3 days.
  2. Before collecting semen it is advisable to urinate and wash genitals so that no bacteria from outer organs would be mixed with the sperm.
  3. The semen has to be collected by masturbation. It must not be collected in a condom.

The sample has to be delivered to our reception centre in Riga, Brīvības gatve 366, or
Daugavpils, (arranging an appointment beforehand by phone 65423125), 18.novembra street 19, from 9:00 – 12:00 within one hour after collecting it and it has to be kept in temperatures between 20° to 40° C.

Collecting 24h urine.


  1. In the morning the patient urinates, but does not collect the urine.
  2. All following portions of urine have to be collected in one container and stored at +2- +8°C.
  3. The next morning the patient urinates and adds the portion to the previous portions.
  4. Then the 24 hour urine amount has to be measured and put down on the testing order.
  5. Then the urine in the container has to be mixed,
  6. and poured in a smaller container ~100 ml.
  7. On the container the patient puts down his/her – name, surname, 24h urine amount, and then it has to be brought to the laboratory

!!! For the following tests:

  • adrenaline;
  • dopamine;
  • noradrenalin;
  • vanillylmandelic acid;
  • metanefrine

Just before collecting the urine, the preservative 30 ml 6N hydrochloric acid has to be poured in the container/cup. You can get both the container and the preservative at the laboratory

Oral glucose tolerance tests.

Preparing the patient

    1. At least 3 days before the testing patient has to keep the usual eating habits (at least 150 – 200g of carbohydrates a day).
    2. At least 3 days before the testing the patient has to stop taking medication that can affect the glucose level in blood (if it does not endanger the patient’s health).
    3. The patient should continue usual physical activities, but also avoid intensive physical loads or prolonged confinement to bed.
    4. These tests should not be done for women while they are having their period.

The procedure of testing

  1. The first blood sample has to be taken in the morning while the patient has not yet eaten. The blood has to be collected in the tube that has stabilizer NaF in it (grey cork; mark 1. tube)
  2. Afterwards in the laboratory the patient has to receive and in 5 minutes time drink glucose dissolved in water.
  3. During the testing (2 hours from the moment that the glucose dilution has been drunk until the next blood sample is collected) the patient has to stay in a calm environment inside the medical facility, without physical activities and eating, and the patient cannot smoke.
  4. The next blood sample is collected after 120 minutes (mark 2. tube)

Collecting urine samples for microbiological testing.

Before a treatment with antibiotics has started, average portion of a urine squirt has to be collected in a sterile container and delivered to the laboratory in 2 hours time for microbiological testing.
If it is not possible it should be stored in a fridge or collected in a special BD VACUTAINER tube that has a special conservant or boric acid (the tube with a partly yellow, partly red or green cork) and delivered to the laboratory in 24 hours time.

Collecting urine samples.

For clinical analysis the morning portion of the urine is used.
In case of need any other portion of the day can also be used (following the correct procedure for collecting it)

    1. The hands and genitals must be washed with warm water

    1. Open the lid of the container. Do not touch the inside of the container. At first urinate a little in the toilet.
    2. After that urinate in the container filling about 2/3 of it.

      1. Pour the urine in the tube with the conservant up to the marker.
        Close the tube with the cork. Deliver the tube to the laboratory.


The urine tube has to be marked with:

  • The name and surname of the patient;
  • The Date and time when the urine was collected.